whole mouse genome dna microarray 4 × 44k ver.2.0 (g4846a)  (Agilent technologies)

Journal: PLoS ONE

Article Title: Somatic Donor Cell Type Correlates with Embryonic, but Not Extra-Embryonic, Gene Expression in Postimplantation Cloned Embryos

doi: 10.1371/journal.pone.0076422

Figure Lengend Snippet: List of samples from E6.5 cloned embryos analyzed by microarray.

Article Snippet: Amplified RNA was labeled with Cy3 dye (GE Healthcare UK Limited, Buckinghamshire, England) and hybridized to a whole mouse genome oligo DNA microarray (4×44 K, Agilent Technologies, Palo Alto, CA, USA) for 17–18 h at 65°C.

Techniques: Clone Assay, Microarray

Journal: PLoS ONE

Article Title: Somatic Donor Cell Type Correlates with Embryonic, but Not Extra-Embryonic, Gene Expression in Postimplantation Cloned Embryos

doi: 10.1371/journal.pone.0076422

Figure Lengend Snippet: ( A ) Embryos were retrieved at E6.5 (left) and dissected into four parts (right). EPC, ectoplacental cone; VE, visceral endoderm; EXE, extraembryonic ectoderm; EPI, epiblast. Scale bar = 100 µm. B) Raw signal values of Pou5f1 (left), Cdx2 (center) and Pgk1 (right) genes extracted from microarray data. Pou5f1 and Cdx2 were detected exclusively in the embryonic and extraembryonic samples, respectively, while Pgk1 was detected in both samples. These results confirmed the accuracy of sample preparation from the embryonic and extraembryonic tissues. Em, embryonic samples; Ex, extraembryonic samples; IVF, samples from in vitro fertilized control embryos; CC, cumulus cell-derived clone; FC, fibroblast-derived clone; SC, Sertoli cell-derived clone. See Figure S2 for further validation by several other marker genes.

Article Snippet: Amplified RNA was labeled with Cy3 dye (GE Healthcare UK Limited, Buckinghamshire, England) and hybridized to a whole mouse genome oligo DNA microarray (4×44 K, Agilent Technologies, Palo Alto, CA, USA) for 17–18 h at 65°C.

Techniques: Microarray, Sample Prep, In Vitro, Derivative Assay, Marker